MersV1, Main, Exploration, bibRecord, 001A74

Structure, diversity and evolution of myriapod hemocyanins.

Identifieur interne : 001A74 ( Main/Exploration ); précédent : 001A73; suivant : 001A75

Structure, diversity and evolution of myriapod hemocyanins.

Auteurs : Christian Pick [Allemagne] ; Samantha Scherbaum ; Elöd Hegedüs ; Andreas Meyer ; Michael Saur ; Ruben Neumann ; Jürgen Markl ; Thorsten Burmester

Source :

The FEBS journal [ 1742-4658 ] ; 2014.

RBID : pubmed:24520955

Descripteurs français

KwdFr :
- Animaux, Arthropodes (), Arthropodes (génétique), Données de séquences moléculaires, Modèles moléculaires, Séquence d'acides aminés, Évolution moléculaire.
MESH :
- génétique : Arthropodes.
- Animaux, Arthropodes, Données de séquences moléculaires, Modèles moléculaires, Séquence d'acides aminés, Évolution moléculaire.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Arthropods (chemistry), Arthropods (genetics), Evolution, Molecular, Hemocyanins (chemistry), Hemocyanins (genetics), Models, Molecular, Molecular Sequence Data.
MESH :
- chemical , chemistry : Hemocyanins.
- chemistry : Arthropods.
- genetics : Arthropods, Hemocyanins.
- Amino Acid Sequence, Animals, Evolution, Molecular, Models, Molecular, Molecular Sequence Data.

Abstract

Oxygen transport in the hemolymph of many arthropods is mediated by hemocyanins, large copper-containing proteins that are well-studied in Chelicerata and Crustacea, but had long been considered unnecessary in the subphylum of Myriapoda. Only recently has it become evident that hemocyanins are present in Scutigeromorpha (Chilopoda) and Spirostreptida (Diplopoda). Here we present evidence for a more widespread occurrence of hemocyanin in the myriapods. By means of RT-PCR, western blotting and database searches, hemocyanins were identified in the symphylans Hanseniella audax and Symphylella vulgaris, the chilopod Scolopendra subspinipes dehaani and the diplopod Polydesmus angustus. No hemocyanins were found in the diplopods Polyxenus lagurus, Cylindroiulus punctatus, Glomeris marginata, Glomeris pustulata and Arthrosphaera brandtii, or the chilopods Lithobius forficatus, Geophilus flavus and Strigamia maritima. This suggests multiple independent losses in myriapod taxa. Two independent hemocyanin subunits were found that were already present in the myriapod stem line. We specifically investigated the structure of the hemocyanin of P. angustus, which consists of three distinct subunits that occur in an approximately equimolar ratio. As deduced by 3D electron microscopy, the quaternary structure is a 3 × 6-mer that resembles the half structure of the 6 × 6-mer hemocyanin from Scutigera coleoptrata. It was analyzed more closely by homology modeling of 1 × 6-mers and their rigid-body fitting to the electron density map of the 3 × 6-mer. In addition, we obtained the cDNA sequence of a putative myriapod phenoloxidase. Phenoloxidases are related to the arthropod hemocyanins, but diverged before radiation of the arthropod subphyla.

DOI: 10.1111/febs.12742
PubMed: 24520955

Affiliations:

Allemagne

Le document en format XML

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<front><div type="abstract" xml:lang="en">Oxygen transport in the hemolymph of many arthropods is mediated by hemocyanins, large copper-containing proteins that are well-studied in Chelicerata and Crustacea, but had long been considered unnecessary in the subphylum of Myriapoda. Only recently has it become evident that hemocyanins are present in Scutigeromorpha (Chilopoda) and Spirostreptida (Diplopoda). Here we present evidence for a more widespread occurrence of hemocyanin in the myriapods. By means of RT-PCR, western blotting and database searches, hemocyanins were identified in the symphylans Hanseniella audax and Symphylella vulgaris, the chilopod Scolopendra subspinipes dehaani and the diplopod Polydesmus angustus. No hemocyanins were found in the diplopods Polyxenus lagurus, Cylindroiulus punctatus, Glomeris marginata, Glomeris pustulata and Arthrosphaera brandtii, or the chilopods Lithobius forficatus, Geophilus flavus and Strigamia maritima. This suggests multiple independent losses in myriapod taxa. Two independent hemocyanin subunits were found that were already present in the myriapod stem line. We specifically investigated the structure of the hemocyanin of P. angustus, which consists of three distinct subunits that occur in an approximately equimolar ratio. As deduced by 3D electron microscopy, the quaternary structure is a 3 × 6-mer that resembles the half structure of the 6 × 6-mer hemocyanin from Scutigera coleoptrata. It was analyzed more closely by homology modeling of 1 × 6-mers and their rigid-body fitting to the electron density map of the 3 × 6-mer. In addition, we obtained the cDNA sequence of a putative myriapod phenoloxidase. Phenoloxidases are related to the arthropod hemocyanins, but diverged before radiation of the arthropod subphyla.</div>
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Structure, diversity and evolution of myriapod hemocyanins.

Structure, diversity and evolution of myriapod hemocyanins.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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